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Figure 8

TCR gene correlations with psoriasis-associated cytokines. () Correlation of gene expression of TRB within psoriatic plaques with psoriasis-associated cytokines in the discovery RNA-Seq dataset (Tsoi et al. dataset details [32] listed in Table 6 ). Spearman correlation coefficients and values are displayed on each plot. values for Spearman’s test were calculated using the asymptotic t approximation. Significant correlations are outlined in blue. () Paired correlation analysis (psoriasis lesion versus matching nonlesion samples) in discovery RNA-Seq dataset. Differences in gene expression were calculated (normalized gene expression of nonlesion samples were subtracted from normalized gene expression of matching lesion samples). Then, the Spearman correlation coefficient was calculated for the difference in gene expression. Correlation coefficients and values are displayed on each plot. values for Spearman’s test were calculated using the asymptotic t approximation. Significant correlations ( < 0.05) are outlined in blue. () Waterfall plots depicting IL-17A correlations for all TRAJ and TRGV gene segments in discovery RNA-Seq dataset. Differences in gene expression were calculated (normalized gene expression of nonlesion samples were subtracted from their corresponding values of matching lesion samples). Then, Spearman correlation coefficients for differences in gene expression were calculated. Spearman correlation coefficients are depicted on the axis. TRAJ or TRGV gene segments are separated on the axis. values for Spearman’s test were calculated using the asymptotic t approximation. Significant correlations ( < 0.05) are highlighted in blue.

Importantly, neither TRB nor TRD correlated with IL-17A ( Figure 8A ). However, this does not rule out T cells (either αβ or γδ) as a major source of this cytokine. One possibility is that other cells, in addition to T cells, secrete IL-17A, making it difficult to detect IL-17A correlations with TRB genes. Another possibility is that only a small fraction of the T cells (either αβ or γδ) secrete IL-17A. In support of the latter possibility, prior groups have demonstrated that T cells bearing certain γδ rearrangements can be skewed toward a Th17 immune response ( 39 ). Thus, we attempted to identify significant correlations between IL-17A expression and the expression of the different TCR variable gene segments; however, none of the correlations identified reached statistical significance ( Supplemental Table 14 ), including those for TRGV5, TRBV3-1, and TRBV3-2.

In contrast, TRAJ23 (lesional versus nonlesional) correlated strongly with IL-17A ( r = 0.63, P = 0.00041, Figure 8, B and C ), and although TRGV5 did not correlate with IL-17A, it did strongly correlate with IL-36A ( r = 0.74, P = 1 × 10 –5 , Figure 8, B and C ), other IL-36 family members, and IL-17C ( r = 0.57, P = 0.002) ( Supplemental Table 15 ).

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12 EGOT Winners (and 25 Almost-EGOTS)
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Life should have been good for Miami Vice ’s Philip Michael Thomas in 1985. He was the star of one of television’s biggest hits , had released his first album as part of a multimillion dollar deal with Atlantic Records, and was making a name for himself in the fashion world (or at least trying to) with his very own women’s clothing line.But Thomas still had loftier goals, both in mind and on the gold medallion he was so fond of wearing. That dream was an EGOT.

Though Thomas swore that the engraved letters E, G, O, and T on his prized necklace stood for energy, growth, opportunity, and talent, those around the then-36-year-old actor unanimously gave a different translation: Emmy, Grammy, Oscar, and Tony—the four awards Thomas had intended to win over the next few years. It’s now more than 30 years later and Thomas has yet to even be nominated for any one of those accolades.

While an EGOT may seem an unlikely reality for Thomas, it’s not an impossibility for all artists. If Fendi floral jacquard knit top Really Low Shipping Sale Online Get Original Cheap Online w0qFXQa
can beat out Benedict Cumberbatch to win this year's Emmy for Outstanding Lead Actor in a Limited Series or a Movie for Jesus Christ Superstar Live in Concert , he'll become the 13th member of the EGOT winners' circle—and one of its youngest.Here are the 12 current members, a couple of SHEGOTS, plus several artists who are just one award away.

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Before there was even a name for it, American composer Richard Rodgers became the first person to EGOT (yes, the acronym can also be used as a verb) when he won an Emmy for the television documentary Winston Churchill: The Valiant Years . His Oscar came in 1945, when his “It Might as Well Be Spring” from State Fair was named Best Song. He earned Grammys in both 1960 and 1962, for the original cast recordings of The Sound of Music and No Strings , respectively. Between 1950 and 1962, he won six Tony Awards, three of them in that first year for South Pacific . The same year, South Pacific also earned Rodgers a Pulitzer Prize for Drama, which we guess makes him a PEGOT.

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In 1977, 15 years after Rodgers inaugurated the honor, actress Helen Hayes joined him as the first female EGOT—an honor that took her 45 years to achieve, the longest of any of her EGOT peers. Her road began in 1932, when she won the Oscar for The Sin of Madelon Claudet (she won a second Oscar for 1970’s Airport) . Her first Tony came in 1947, for Happy Birthday , followed by another in 1958 for Time Remembered . And she won a Best Actress Emmy in 1953 for an episode of Schlitz Playhouse of Stars . But it would take more than two decades for her to nab that elusive second letter, which she did for Best Spoken Word Recording for Great American Documents.

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Journal of Neural Engineering

Jingtao Huang , Mesut Sahin and Dominique M Durand

Published 15 August 2005 2005 IOP Publishing Ltd Journal of Neural Engineering , Volume 2 , Number 4

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sahin@coes.latech.edu

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1 Center for Biomedical Engineering and Rehabilitation Science, Department of Biomedical Engineering, Louisiana Tech University, Ruston, LA 71270, USA

2 Neural Engineering Center, Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA

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Received 4 February 2005 Accepted 10 June 2005 Published 15 August 2005

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Jingtao Huang et al 2005 J. Neural Eng. 2 73

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The functional effects of selective hypoglossal nerve (HG) stimulation with a multi-contact peripheral nerve electrode were assessed using images of the upper airways and the tongue in anesthetized beagles. A biphasic pulse train of 50 Hz frequency and 2 s duration was applied through each one of the tripolar contact sets of the nerve electrode while the pharyngeal images were acquired into a computer. The stimulation current was limited to 20% above the activation threshold for maximum selectivity. The images showed that various contact sets could generate several different activation patterns of the tongue muscles resulting in medial and/or lateral dilation and closing of the airways at the tongue root. Some of these patterns translated into an increase in the oropharyngeal size while others did not have any effect. The pharyngeal sizes were not statistically different during stimulation either between the two different positions of the head (30° and 60°), or when the lateral contacts were compared with the medial ones. The contacts that had the least effect generated an average of 53 ± 15% pharyngeal dilation relative to the best contacts, indicating that the results are marginally sensitive to the contact position around the HG nerve trunk. These results suggest that selective HG nerve stimulation can be a useful technique to produce multiple tongue activation patterns that can dilate the pharynx. This may in turn increase the size of the patient population who can benefit from HG nerve stimulation as a treatment method for obstructive sleep apnea.

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