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Though John Stevens didn’t confirm his availability, Jake Muzzin will be expected to make his return to the LA Kings lineup for tonight’s critical Game 3 against a Vegas Golden Knights team that has a two-to-nothing lead in the teams’ best-of-seven first round series.

Muzzin hasn’t played since suffering an upper-body injury during Los Angeles’ 3-0 win over Calgary on March 26. He etched out perhaps his finest individual season as a pro, posting eight goals and a career-high 42 points in 74 games and leading team regulars with a 52.9% Corsi-for percentage.

“I think he’s really close. I think he’s going to be a game-time decision,” Stevens said. “But we were just joking there – Muzz, he’s begging to stop getting a conditioned skate every day, so we we thought we’d get him in a regular rotation today. He’s working really hard. I think he’s close, so we’ll see. I haven’t talked to the trainer yet, but I’ll make sure to talk to him and see where he’s at, and then we’ll go from there.”

Muzzin skated to the left of Drew Doughty, who did not play in Game 2 due to an NHL-imposed suspension for an illegal check to the head of Golden Knights forward William Carrier in Game 1. Though Doughty had skated primarily with Derek Forbort (who remains out with a lower-body injury) in the second half of the season and Muzzin with Christian Folin, their reunion would reprise a partnership that provided excellent minutes during the Kings’ Stanley Cup run of 2014. Again, Stevens did not confirm Muzzin’s place in the lineup, but he did confirm that he has been cleared for contact, and Muzzin indicated he’d play Sunday night.

“Yeah it’s been a long time,” he said. “I’m excited. You know, it’s tough to watch, … but I’m excited to get back in and try and help the guys win.”

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John Stevens, on the potential of having Drew Doughty and Jake Muzzin back for Game 3: I thought we had some guys come in and really step up big. I think oscar’s come in and done a really good job. But with Drew in your lineup, you’ve got a guy that plays big minutes in all situations that can really control the pace of the game, and I think beyond Drew as a player, he’s one of the leaders on our hockey team. The amount that he’s grown as a leader on our team has far surpassed his improvement as a player, and I think that’s been huge for our team. I’ve said this many times; he’s the most competitive guy that I think I’ve ever seen. To add that back to your lineup for us is just good for the group.

Winterfest in Kennebunk

January 27 FROSTED! a freezing good time at7pm at The Boathouse Watefront Hotel – the kick-off event of Kennebunkport’s Paint the Town Red month, this 4th Annual Ice Bar party is Saturday, tickets are $55 in advance , forice bar with luge, light snacks, s’mores hot chocolate bars, entertainment, and two signature drink tickets.

January 27 FROSTED!

Feb 3 Bubbles Truffles with Chef Pierre Gignac –Join theChef from Ocean at Cape Arundel Inn, for sparkling wine and chocolate truffles, $40 pp, held at Grand Hotel 1-3pm.

Feb 3 Bubbles Truffles with Chef Pierre Gignac

Feb 3 TASTES OF ITALY + WINE PAIRING 3-5 at Ports of Italy . Chef German Luca shares four wines with travel tasting tips. $45

Feb 3 TASTES OF ITALY + WINE PAIRING

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$50 per person, includes six 3oz wine pours, light appetizers.

Feb 10 Soup Snow Stroll 9-11 Join Kennebunk Land Trust, Boulangerie, a Proper Bakery, and Ramblers Way for a snow shoe or walk at the Mousam River Wildlife Sanctuary then enjoy a warm drink, a cup of soup and sweets at Boulangerie.

Feb 10 Soup Snow Stroll

Feb 10 Wines Valentines 1-3, at Kennebunkport Inn enjoy wine tastings small plates with the culinary team at The Burleigh, $15.

Feb 10 Wines Valentines

Feb 10 MAKE THE PERFECT COCKTAIL 1-3 , Old Vines Wine Bar. $60 per person, includes three cocktails, light appetizers, reserve in advance. Old Vines Wine Bar .

Feb 10 MAKE THE PERFECT COCKTAIL

Feb 10 Date Skate 4-6 Skating at the Waterhouse Center with a live DJ.

Feb 10 Date Skate

Feb 10 Earth at Hidden Pond Pop Up Winter Dinner at 6, at Lodge on the Cove – Chef Joe Shafer debuts Earth’s 2018 menu in a Three-Course family-style dinner, $125.

Feb 10 Earth at Hidden Pond Pop Up Winter Dinner

Feb 10 TASTES OF ITALY + WINE PAIRING 3-5 at Ports of Italy . Chef German Luca shares four wines with travel tasting tips. $45

Feb. 13 14 FRENCH FAMILY DINNER 6 – 9at Old Vines Wine Bar . Chef Joel and his team will prepare a classic French four-course dinner, served family-style. $79.

Feb 15 Alisson’s Restaurant Choose Your Own Adventure Beer Dinner , with Orono Brewing Company t a do-what-you-want beer dinner! Offering Orono brews with suggested food pairings, all a la carte.

Feb 17 Drinking through the Decades1-3, at Kennebunkport Inn – learn to make five decades of cocktails at The Burleigh, $40.

Fig 3. Detection of GFP-expressing BCG by innate cells and association between absolute numbers of innate cells and mycobacterial growth inhibition.

( A ) Representative flow cytometry plot of IL-6, IL-12 and TNF-α cytokine expression by myeloid dentritic cells (mDCs), monocytes and neutrophils, measured in whole blood stimulated for 6 hours with BCG, BCG-GFP (shown) or LPS, relative to an unstimulated control sample. ( B ) Representative histograms indicating proportions of innate cells that phagocytosed BCG-GFP (green). ( C ) Absolute numbers of innate cell subsets per milliliter of unstimulated whole blood plotted against M . tb H37Rv growth. R and p values were calculated using Spearman’s correlation. ( D ) Absolute numbers of BCG-GFP-positive mDCs, monocytes or neutrophils per mL of whole blood in adult individuals, stratified by QFT status. The inclusion of TruCount beads during the cell staining steps of the innate whole blood assay allowed determination of the absolute number of each subset of cells per mL of whole blood. The red and blue circles represent QFT+ and QFT- adults, respectively. Horizontal lines represent medians and whiskers, the interquartile range. Differences in absolute counts of BCG-GFP-positive innate cells between the groups were evaluated with the Mann-Whitney test (shown P values). The pie charts show relative proportions of BCG-GFP-positive cells among each innate cell subset.

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Finally, we determined if absolute counts of BCG-GFP-specific innate cells co-expressing IL-6, IL-12 and/or TNF-α were associated with control of M . tb growth. Again, no correlation between absolute numbers of total cytokine expressing innate cells and control of mycobacterial growth was observed ( Fig 4A ). Further, there was no difference in numbers of mDCs, monocytes and neutrophils expressing specific patterns of innate cytokines between QFT+ and QFT- adults ( Fig 4B ).

Fig 4. Mycobacteria-specific cytokine expression by innate cells in whole blood from adults and mycobacterial growth inhibition.

( A ) Absolute numbers of total cytokine expressing innate cells per mL of whole blood plotted against M . tb H37Rv growth. R and p values were calculated using Spearman’s correlation analysis. ( B ) Numbers of BCG-specific mDCs, monocytes and neutrophils co-expressing IL-6, IL-12 and/or TNF-α in whole blood from QFT+ (red) and QFT- (blue) adults. Medians are represented by the horizontal line, interquartile ranges by the boxes, and ranges by the whiskers. The Mann-Whitney test was used to assess differences between QFT+ and QFT- adults and none were found to be different.

Primary Ciliary Dyskinesia (PCD, OMIM #242650 ) or Immotile Cilia Syndrome (ICS) is an hereditary condition affecting the motility of ciliary and spermatozoa flagella. The disorder, transmitted in an autosomal recessive mode, has an incidence of 16,000-30,000. Dysmotility to complete immotility of cilia and flagella results in multisystemic disease of varying severity, characterized by infections of the upper respiratory tract, bronchiectasis, and male sterility. When heterotaxia (situs inversus) is associated, as in in up to half of patients, PCD is also referred to as Kartagener syndrome ( OMIM #244400 ). The condition is most often associated with ultrastructural defects of axoneme, the skeleton of cilia and flagella, which is constituted of several families of proteins including dyneins, kinesin and microtubule-associated proteins (MAPs).

Our recent genome-wide linkage studies in over 30 families identified several chromosomal regions which may anchor genes linked to the phenotype, suggesting that the disease is genetically highly heterogeneous1. These results, were compatible with the localization of several dynein genes, long standing candidates for PCD since roughly half of the structural defects are in the dynein arm. Indeed, in the last few months, we and others have found mutations in 3 distinct axonemal dynein genes (DNAI1, DNAH11, DNAH5) in Kartagener syndrome. Since each of these mutations was observed in only few families thusfar, other genes, probably including additional isoforms of dynein, must also be responsible for PCD. Multiple dynein proteins are encoded by a family of distinct genes mapping to different chromosomes.

( Blouin et al. 2000 )

Call to collaboration

Periodical Bulletin on the scientific research on Primary Ciliary Dyskinesia at Medical Genetics

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in the identification of disease-causing genes, November 2001. This bulletin was sent to physicians who collaborated to our research project on PCD. This document provide informations also that physicians are encouraged to forward to the patients and families who where included in the study.

A list of publications on Kartagener Syndrome Project by the group.

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of the University of Geneva Medical School and collaborators.

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